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研制了一种基于激光诱导荧光检测方法的微流控芯片分析仪。该分析仪使用玻璃基质聚二甲基硅氧烷(PDMS)微流控芯片,可一次性进行12通道的电泳分离实验。仪器采用共聚焦式光路结构,并可通过检测由微流控芯片反射的激光信息,控制步进电机实现芯片的自动精确定位。实验结束自动保存数据,绘制分离图谱。。对9种不同长度的50 bp DNA Ladder片段进行电泳分离及数据分析,耗时在5 min内,且分离效果良好。
A microfluidic chip analyzer based on laser-induced fluorescence detection was developed. The analyzer uses a glass matrix polydimethylsiloxane (PDMS) microfluidic chip, can be a one-time 12-channel electrophoretic separation experiments. The instrument uses a confocal optical path structure, and can detect the laser information reflected by the microfluidic chip to control the stepping motor to realize the automatic and accurate positioning of the chip. The end of the experiment automatically save the data, drawing separation map. . Electrophoretic separation and data analysis of nine different lengths of 50 bp DNA Ladder fragments took about 5 min and the separation efficiency was good.