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目的:观察早期贲门腺癌(GCA)中SOX17基因启动子区甲基化状态,探索其与GCA肿瘤发生进展之间的关系,及SOX17基因甲基化作为早期GCA预后诊断标记物的可能性。方法:收集早期GCA肿瘤组织、癌旁正常组织以及癌旁正常淋巴结,采用基于磁珠的DNA提取和亚硫酸氢钠修饰技术,结合实时荧光定量PCR技术检测SOX17基因启动子区甲基化状态。结果:早期GCA肿瘤组织中SOX17基因启动子区甲基化阳性率为73.8%,正常组织中为4.9%,GCA肿瘤组织中SOX17甲基化发生率明显高于正常组织(P<0.001),但与疾病预后无关(P=0.14)。有18例同时在肿瘤组织和淋巴结中检测到SOX17启动子区甲基化。生存分析显示,淋巴结中SOX17甲基化与GCA疾病预后有关(P<0.05)。结论:早期GCA肿瘤组织和淋巴结中均可检测到SOX17基因启动子区的异常甲基化状态。淋巴结中检测SOX17启动子甲基化作为早期GCA肿瘤预后判断的分子标记物具有重要临床意义。
OBJECTIVE: To observe the methylation status of SOX17 promoter in gastric cardia adenocarcinoma (GCA) and to explore its relationship with the progression of GCA tumor and the possibility of methylation of SOX17 gene as a diagnostic marker for early GCA prognosis. Methods: Early GCA tumor tissues, adjacent normal tissues and adjacent normal lymph nodes were collected. The methylation status of SOX17 promoter region was detected by using DNA-based DNA extraction and sodium bisulfite modification combined with real-time fluorescence quantitative PCR. Results: The positive rate of SOX17 promoter methylation was 73.8% in early GCA tumor tissues and 4.9% in normal tissues. The incidence of methylation of SOX17 in GCA tumor tissues was significantly higher than that in normal tissues (P <0.001) Not related to disease prognosis (P = 0.14). In 18 cases, SOX17 promoter methylation was detected in both tumor tissues and lymph nodes. Survival analysis showed that SOX17 methylation in lymph nodes was associated with prognosis of GCA disease (P <0.05). Conclusion: The aberrant methylation status of SOX17 gene promoter region can be detected in both early GCA tumor tissues and lymph nodes. Detection of SOX17 promoter methylation in lymph nodes is of great clinical significance as a molecular marker for early prognosis of GCA tumors.