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在恶性肿瘤的治疗中,国外目前应用强烈化疗或放疗,以期达到根治之目的.但其严重的骨髓抑制大大地限制了它的应用.自身骨髓移植就是在化疗前从人体内抽出,并在体外保存一段时间,然后待药物的毒性作用消失再将其回输入体内.这就避免了上述的副作用.因此能否有效地保存细胞,维持其活力和功能是自身骨髓移植的关键.本文介绍了骨髓的保存方法,尤其是超低温保存.取得的结果是令人满意的.人骨髓于4℃可有效地保存3天,有核细胞、锥蓝拒染率和粒系定向干细胞(CFU—C) 的回收率分别为84%、91%和97%.骨髓贮存于液氮中,其细胞的活力和功能得以较长保存,有核细胞、锥蓝拒染率、脱氧核糖核酸(DNA)和CFU—C的回收率分别为76%、89%、77%和79%.骨髓造血干细胞在体外成功的保存,为临床开展自身骨髓移植提供了先决条件.
In the treatment of malignant tumors, the current foreign application of intensive chemotherapy or radiotherapy, in order to achieve the purpose of cure, but its severe myelosuppression greatly limits its application.Material bone marrow transplantation is extracted from the human body before chemotherapy and in vitro Save for a period of time, and then wait for the disappearance of the toxic effects of drugs and then back into the body .This avoids the above side effects.Therefore, the effective preservation of cells, to maintain their vitality and function is the key to their own bone marrow transplantation.This article describes the bone marrow , Especially cryopreservation, the results obtained are satisfactory.The human bone marrow can be efficiently stored at 4 ° C for 3 days, with nucleated cells, blue cone staining and granulocyte-directed stem cells (CFU-C) The recovery rate of bone marrow was 84%, 91% and 97%, respectively.The bone marrow was stored in liquid nitrogen and its cell viability and function were preserved for a long time.The number of nucleated cells, Cone blue dye rejection rate, DNA and CFU- The recovery rate of C was 76%, 89%, 77% and 79%, respectively.The successful preservation of bone marrow hematopoietic stem cells in vitro provided the precondition for clinical transplantation of bone marrow.