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目的:考察梓醇对脑缺血大鼠髓鞘的保护作用。方法:采用大鼠大脑中动脉缺血模型,将造模大鼠分成模型组、梓醇15mg/kg、30mg/kg、60mg/kg组、丁苯酞70mg/kg组和依达拉奉7mg/kg组和假手术组,缺血后3d至14d给药。采用Longa法对大鼠神经功能进行评分;HE染色和卢卡斯快蓝(luxolfastblue,LFB)染色观察胼胝体区髓鞘改变;免疫组织化学检测胼胝体髓鞘碱性蛋白(myelinbasicprotein,MBP)表达。结果:与假手术组比较,模型组大鼠的神经功能明显损伤;梓醇30mg/kg、60mg/kg组大鼠神经功能行为学评分明显降低。HE染色,模型组可见胼胝体白质疏松、空泡形成、胶质细胞深染、间质水肿;梓醇组胼胝体白质少许空泡形成、胶质细胞形态趋于正常。LFB染色,模型组髓鞘颜色变浅,髓鞘变得稀疏;梓醇30mg/kg、60mg/kg组积分光密度值显著升高。MBP蛋白在假手术组的表达较强,为黄褐色;在模型组表达明显减弱,着色较浅,且分布不均匀;梓醇30mg/kg、60mg/kg组MBP积分光密度值显著升高。结论:梓醇可促进大鼠脑缺血损伤后神经功能障碍的恢复,可能与髓鞘的保护作用有关。
Objective: To investigate the protective effect of catalpol on myelin of cerebral ischemic rats. Methods: Rat model of middle cerebral artery occlusion was established. The model rats were divided into model group, catalpol 15mg / kg, 30mg / kg, 60mg / kg group, butylphthalide 70mg / kg group and edaravone 7mg / kg group and sham operation group, 3d to 14d after ischemia. The neurological function of rats was assessed by Longa method. The myelin sheath was observed by HE staining and luxolfast blue (LFB) staining. The expression of myelin basic protein (MBP) was detected by immunohistochemistry. Results: Compared with the sham operation group, the neurological function of the model group was obviously damaged. The neurological behavioral score of rats in 30 mg / kg and 60 mg / kg groups significantly decreased. HE staining, the model group showed corpus callosum leukoaraiosis, vacuolar formation, glial cells deep staining, interstitial edema; catalpol group corpus callosum white matter vacuoles formation, glial cell morphology tends to normal. LFB staining, the myelin sheath became lighter and the myelin became thinner. The integral optical density values of catalpol 30mg / kg and 60mg / kg were significantly increased. The expression of MBP protein in the sham-operated group was stronger than that in the sham operation group. The expression of MBP protein in the model group was significantly weakened, the staining was shallow and the distribution was not uniform. The MBP integral optical density value of 30mg / kg and 60mg / kg groups significantly increased. Conclusion Catalpol can promote the recovery of neurological dysfunction after cerebral ischemic injury in rats, which may be related to the protective effect of myelin.