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建立叶酸靶向复合脂质体中阿霉素和维拉帕米含量测定的反相高效液相色谱法。阿霉素在0.5—60μg/mL范围内呈良好线性关系,平均回收率97.3%;维拉帕米在1.0—50.0μg/mL范围内呈良好线性关系,平均回收率97.2%。本方法灵敏度高,专属性强,重现性好,操作简便,能准确测定叶酸靶向复合脂质体中阿霉素和维拉帕米的含量。
To establish a reversed phase HPLC method for the determination of doxorubicin and verapamil in folate-targeted liposomes. Doxorubicin showed good linearity in the range of 0.5-60μg / mL with an average recovery of 97.3%. Verapamil showed a good linearity in the range of 1.0-50.0μg / mL with an average recovery of 97.2%. The method has the advantages of high sensitivity, specificity, good reproducibility, simple operation, accurate determination of doxorubicin and verapamil in the folate-targeted liposome.