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Objective: To investigate the effect of MEK1 inhibitor PD98058 on Tec and ERK2 in HepG2 hepatoma cells.Methods: The expression of mRNA and protein of Tec and ERK2 in HepG2 cells was detected by immunocytochemistry assay. After various concentration of PD98059 treatment, the expression of Tec and ERK2 mRNA in HepG2 cells was detected by RT-PCR and Westem blotting. Results: Tec and ERK2 expressed highly in HepG2 cells. PD98059 obviously inhibited the expression of mRNA and protein of Tec and ERK2 in a dose-dependent manner, in which 40 μmol/L of PD98059 exhibited the strongest inhibiting effect. Conclusion: PD98058, as MEK1 inhibitor, can inhibit Tec, block the signal route of Ras/Raf/ERK and to impede the signal transduction in HepG2 cells. Tec may be the signal protein in the upper stream of Ras/Raf/ERK in hepatocarcinoma cells and is supposed to interact with the signal way of Ras/Raf/ERK.