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目的:探讨降香药材质量的液相色谱指纹图谱分析方式。方法:使用梯度洗脱的方式做色谱分离,借助计算机辅助相似度的判断软件做有效的数据处理,并对不同的降香药材指纹图谱相似度做对比分析。结果:依照指纹图谱相似度的分析结果,把降香药材分成三大类,同时在三大类降香药材中认定了10个、7个以及2个酚性成分。结论:该方法可应用于降香药材的指纹图谱测定,并且为质量判定提供有效参照。
Objective: To investigate the quality of medicinal herbs by HPLC fingerprint analysis. Methods: The method of gradient elution was used for chromatographic separation. Computer-aided similarity software was used to do effective data processing. The fingerprint similarity of different medicinal herbs was comparatively analyzed. Results: According to the similarity analysis of fingerprints, the medicinal herbs were divided into three categories. At the same time, 10, 7 and 2 phenolic compounds were identified among the three categories of medicinal herbs. Conclusion: This method can be applied to the fingerprint determination of medicinal herbs and provide an effective reference for quality judgment.