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目的综述组织工程细胞片构建方法的研究进展并展望前景。方法广泛查阅近年来有关构建组织工程细胞片的研究与应用的文献,选择常见方法分别进行阐述。结果通过组织工程细胞片方法构建的组织,可以打破传统组织工程方法对组织工程发展的限制。目前构建组织工程细胞片的方法主要包括温敏培养皿、胶原凝胶、磁力组织工程、表面粗糙的颗粒单分子层及聚合电解质。结论 组织工程细胞片构建方法可行,具有广泛的应用前景。
Objective To summarize the research progress and prospect of tissue engineering cell sheet construction method. Methods Extensive review of the literature on the research and application of the construction of tissue engineering cell sheets in recent years, the choice of common methods are described separately. Results The tissue constructed by the method of tissue engineering cell sheet can break the restriction of the traditional tissue engineering method on the development of tissue engineering. At present, the methods of constructing tissue engineered cell sheet mainly include temperature sensitive culture dish, collagen gel, magnetic tissue engineering, coarse particle surface monolayer and polyelectrolyte. Conclusion Tissue engineering cell sheet construction method is feasible and has a wide range of applications.