采用花粉管通道技术将抗赤星病烤烟ＣＶ８７的总ＤＮＡ导入感病烤烟ＮＣ８９，对其Ｄ_１代植株进行了田间生长性状、抗病性和烟叶品质的检测。结果显示，柱头滴加（ＤＺ）、切柱头滴加（ＱＤ）和子房注射（ＺＺ）等３种导入方法得到的Ｄ_１代植株在株高、株型、叶型、生育期等方面发生了较大的变异，变异率分别是１９．１０％，２０％和１８．２０％。３类植侏中分别有９．１０％，１０％和６．４０％的个体获得了对赤星病的抗性。在这些植株中，筛选出ＤＺ９８１１和ＱＤ９８１２在抗病性和烟叶品质上为优良植株。 The pollen tube pathway was used to introduce the total DNA of resistant tobacco CV87 to the infected tobacco NC89. The growth traits, disease resistance and tobacco quality of D_1 plants were tested. The results showed that the D_1 plants obtained by three kinds of introduction methods, such as DZ, QD and ZZ, had higher plant height, plant type, leaf type and growth period Large variation, mutation rates were 19.10%, 20% and 18.20% respectively. 9.10%, 10% and 6.40% of the individuals in the three species of juvenile plants, respectively, were resistant to brown spot disease. Among these plants, DZ9811 and QD9812 were screened to be excellent plants in disease resistance and tobacco quality.