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目的探索模拟失重下空间诱变大肠杆菌(T1-13)感染巨噬细胞RAW264.7后差异表达的microRNAs(miRNAs)及其意义。方法采用第二代测序技术检测模拟失重染菌组与对照组巨噬细胞miRNAs差异表达,通过生物信息学预测差异miRNAs的靶基因,并对靶基因进行GO功能富集分析、KEGG信号通路分析以及STRING蛋白互作分析。结果模拟失重染菌组与对照组间筛选出33个差异miRNAs,下调23个,上调10个。这些miRNAs互补结合的靶基因参与了MAPK通路、WNT通路、轴突导向以及TGF-β等信号通路,其中关键下调的靶基因是MAPK9,MAPK14,MAP3K7,MAP3K14,PIK3CA,PIK3CB,PIK3CD,MYC,JUN,SMAD4;关键上调的靶基因是AKT3,MAPK8,MAPK9,MAPK10,MAP2K1,PIK3CD。结论模拟失重下空间诱变大肠杆菌感染巨噬细胞后miRNAs表达出现显著差异,这些miRNAs可能通过调控MAPK,WNT以及TGF-β等通路参与失重条件下炎症反应的发生。
Objective To explore the differential expression of microRNAs (miRNAs) after RAW264.7 macrophages infected with space-mutated Escherichia coli (T1-13) under simulated weightlessness. Methods The second generation sequencing technique was used to detect the differential expression of miRNAs in macrophages in simulated weightlessness and control groups. Bioinformatics methods were used to predict the target genes of different miRNAs. GO gene enrichment analysis, KEGG signal pathway analysis STRING protein interaction analysis. Results 33 differential miRNAs were screened from the simulated weightlessness-infected group and the control group, down-regulated by 23 and up-regulated by 10. The target genes complementary to these miRNAs are involved in the MAPK pathway, WNT pathway, axon guidance and TGF-β signaling pathway, among which the key target genes are MAPK9, MAPK14, MAP3K7, MAP3K14, PIK3CA, PIK3CB, PIK3CD, MYC, JUN , SMAD4; the key up-regulated target genes are AKT3, MAPK8, MAPK9, MAPK10, MAP2K1, PIK3CD. Conclusions There are significant differences in the expression of miRNAs after E. coli infection of macrophages under simulated weightlessness. These miRNAs may play an important role in the inflammatory response under weightlessness through the regulation of MAPK, WNT and TGF-β.