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目的:从人工蛹虫草子实体中提取游离型麦角甾醇,并测定其含量。方法:采用正交设计法筛选走角甾醇最佳提取条件,用HPLC法测定麦角甾醇含量。结果:走角甾醇在5.8～58.0μg·ml~(-1)范围内线性关系良好(r=0.9999),平均加样回收率为99.5%(RSD=1.3%)。结论:该提取方法快速、简便,结果准确、可靠。
Objective: To extract free ergosterol from artificial Cordyceps militaris fruit body and determine its content. Methods: Orthogonal design was used to screen the optimal extraction conditions for gosberol, and ergosterol content was determined by HPLC. RESULTS: The linear relationship was good in the range of 5.8～58.0 μg·ml -1 (r=0.9999). The average recovery rate was 99.5% (RSD=1.3%). Conclusion: The extraction method is rapid, simple and accurate and reliable.