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目的探讨宫颈癌患者外周血辅助T细胞1(Th1)和辅助T细胞2(Th2)型细胞因子的表达状况。方法 30例宫颈癌患者采集外周血,采用流式细胞分析法,检测患者外周血CD4+细胞胞内细胞因子干扰素γ(IFN-γ)和白介素4(IL-4)的表达。应用酶联免疫吸附方法(ELISA)检测培养上清IFN-γ、IL-4的水平,并与10例健康对照组进行比较。结果宫颈癌患者IFN-γ染色阳性(Th1)的细胞比例及培养上清IFN-γ浓度明显低于健康对照组,IL-4染色阳性(Th2)细胞比例及培养上清IL-4浓度在两组间差异无统计学意义。结论宫颈癌患者存在Th1/Th2细胞功能失衡,呈Th2优势应答模式,机体免疫抑制,导致肿瘤发生,同时为宫颈鳞癌的生物治疗提供靶点。
Objective To investigate the expression of helper T cell 1 (Th1) and helper T cell 2 (Th2) cytokines in peripheral blood of patients with cervical cancer. Methods Thirty patients with cervical cancer were collected peripheral blood and the expression of cytokines IFN-γ and IL-4 in CD4 + cells were detected by flow cytometry. The levels of IFN-γ and IL-4 in culture supernatants were detected by enzyme-linked immunosorbent assay (ELISA) and compared with 10 healthy controls. Results The proportion of IFN-γ-positive cells (Th1) and the concentration of IFN-γ in culture supernatant of cervical cancer patients were significantly lower than those of healthy controls. The proportion of IL-4 positive cells (Th2) No significant difference between groups. Conclusion There is a imbalance of Th1 / Th2 cell function in cervical cancer patients, showing Th2 dominant mode of response, immunosuppression in the body, leading to tumorigenesis and targeting of biological treatment of cervical squamous cell carcinoma.