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为解决竹红菌甲素(hypocrellin A,HA)易溶于有机溶剂却极难溶于水的问题,采用物理包埋法,以Triton X-100为载体,制备了对水稀释后稳定性良好的500 mg/L的竹红菌甲素Triton X-100微乳剂,并测定了其光热稳定性及对植物病原菌的抑制活性。结果表明:在0~54℃、照度低于3 600 lx时,该微乳剂的光热稳定性良好;在照度为7 200 lx时,50 mg/L的HA-TX-100微乳剂对番茄灰霉病菌Botrytis cinerea、松针褐斑病菌Lecanosticta acicola、油菜菌核病菌Sclerotinia sclerotiorum和小麦赤霉病菌Fusarium graminearum的抑制率均超过50%,其中对油菜菌核病菌的抑制率最高,达77.60%;1 000 mg/L的HA-TX-100微乳剂对活体番茄接种番茄灰霉病菌的保护作用与治疗作用抑制率分别为72.05%和64.73%,均优于相同条件下市售50%多菌灵可湿性粉剂的作用。研究结果表明,竹红菌甲素具有开发为光活化农药的潜力。
In order to solve the problem that hypocrellin A (HA) is very soluble in organic solvents, it is extremely difficult to dissolve in water. The physical embedding method and the Triton X-100 Of 500 mg / L Hypocrellin Triton X-100 microemulsion, and its light and heat stability and inhibition of plant pathogenic bacteria was determined. The results showed that the light-heat stability of the microemulsion was good when the illuminance was less than 3 600 lx at 0-54 ℃. When the illumination intensity was 7200 lx, the effect of 50 mg / L HA-TX-100 microemulsion on tomato ash The inhibitory rates of Botrytis cinerea, Lecanosticta acicola, Sclerotinia sclerotiorum and Fusarium graminearum were over 50%, and the highest inhibition rate was 77.60% on rape Sclerotinia sclerotiorum; mg / L of HA-TX-100 microemulsion on Botrytis cinerea inoculated with tomato was 72.05% and 64.73%, respectively, which were better than the 50% carbendazim wettable under the same conditions The role of powder. The results show that Hypocrellin A has the potential to be developed as a photoactivated pesticide.