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采用聚合酶链反应（ＰＣＲ）和限制性片段长度多态性分析（ＲＦＬＰ）技术，对４ｌ例大肠癌组织大肠癌缺失基因杂合性缺失进行研究。结果表明，３８例大肠癌信息个体中有２１例（５５．３％）发现ＤＣＣ基因杂合缺失。ＤＣＣ基因杂合性缺失与肿瘤大小、组织学类型和浸润深度无关，但有淋巴结转移（８０．００％）和ＤｕｋｅｓＣ、Ｄ期（７１．４％）大肠癌Ｄｃｃ基因杂合缺失率显著高于无淋巴结转移（３９．１％）和ＤｕｋｅｓＡ、Ｂ期组（３５．３％，Ｐ＜０．０５）。结果提示，ＤＣＣ基因杂合缺失多发生于大肠癌的晚期，ＤＣＣ基因杂合性缺失的检测有可能成为大肠癌病人预后评价的指标。
Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) techniques were used to study the loss of heterozygosity of colorectal carcinoma deletion genes in 41 cases of colorectal cancer. The results showed that 21 cases (55.3%) of 38 cases of colorectal cancer information were found to have a heterozygous deletion of DCC gene. The loss of heterozygosity of DCC gene was not associated with tumor size, histological type and depth of invasion, but the rate of Dcc gene deletion was significantly higher in patients with lymph node metastasis (80.00%) and DukesC, D phase (71.4%). No lymph node metastasis (39.1%) and DukesA, B phase group (35.3%, P<0.05). The results suggest that DCC gene loss occurs in the late stage of colorectal cancer. The detection of DCC gene loss may be an indicator of prognosis in patients with colorectal cancer.