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验证磁悬浮离心式左心辅助装置—ChinaHeart VAD的在体血液相容性和可靠性,评估其对实验动物主要终末器官的影响。通过6例健康绵羊的在体存活实验进行评估。在心脏不停跳的情况下,装置入口插入左心尖,出口与降主动脉吻合。术前和术后做血常规、生化及血凝检查,术后连续监测动物存活及辅助装置运行状况。择期动物安乐死后,进行终末主要脏器的宏观和病理学检查。4只羊术后3～28 h内因麻醉或手术意外死亡;2只羊成功复苏并分别辅助20 d和38 d后施行安乐死。对于成功存活的2例实验动物,术后护理期间血常规、生化和血凝检查结果均在正常范围内,辅助装置无机械故障发生。尸检时1只羊(存活20 d)血泵泵体内发现一肉眼可见小块血栓沉积,其余无血栓形成;组织病理检查发现2只羊左肺贴近辅助装置部位均可见有小叶性肺炎,其余终末脏器无明显病变发生。ChinaHeart VAD在体辅助期间血液相容性能良好,对各主要终末脏器无显著影响,具有较好的可靠性;动物存活实验中麻醉呼吸管理和手术操作也至关重要。
To verify in-vivo hemocompatibility and reliability of the magnetic levitation-assisted left heart accessory device, China Heart VAD, and assess its effect on the main terminal organ of experimental animals. In vivo survival experiments were performed on 6 healthy sheep. In the case of heart beating, the device inlet is inserted into the left apex, and the exit anastomoses to the descending aorta. Preoperative and postoperative blood tests, biochemistry and hemagglutination, postoperative continuous monitoring of animal survival and auxiliary device operating conditions. Elective animal euthanasia, the terminal macroscopic and pathological examination of major organs. Four sheep were accidentally killed by anesthesia or surgery within 3-28 h postoperatively. Two sheep recovered successfully and were assisted by euthanasia for 20 d and 38 d, respectively. For the 2 surviving experimental animals, blood routine examination, biochemistry and blood coagulation examination results were within the normal range during the postoperative nursing care, and the auxiliary device had no mechanical failure. At the time of autopsy, only 1 sheep (surviving for 20 days) showed a small thrombus deposition in the blood pump pump and the rest without thrombosis. Histopathological examination revealed lobular pneumonia in the left lung of the two animals close to the auxiliary device. End organ no obvious lesions occur. ChinaHeart VAD has good blood compatibility during the body-assisted period, has no significant effect on the major terminal organs and has good reliability. During the animal survival experiment, anesthesia respiratory management and operation are also crucial.