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应用增殖抑制方法发现,无生长抑制作用的0.5mg ·L~(-1)的蝙蝠葛碱(Dau)使博莱霉素A_5单独对小鼠肉瘤S-180V细胞作用的I_(C50)值从2.1mg·L~(-1)降低到1.3mg·L~(-1),无抑制作用的1 mg·L~(-1) Dau使博莱霉素A_5单独对人喉癌HEP2细胞作用的I_(C50) 值从0.33 mg·L~(-1)降低到0.1 mg·L~(-1).克隆形成法证明:无细胞毒性的10 mg·L~(-1)的Dau明显增强博莱霉素A_5对HEP2细胞毒性.TFP法证实,有一定生长抑制作用的Dau对HEP2细胞内活化钙调素有影响.结果表明:蝙蝠葛碱有一定的增强博莱霉素A_5对肿瘤细胞的毒性.
According to the method of proliferation inhibition, I_ (C50) value of the effect of bleomycin A_5 alone on mouse sarcoma S-180V cells was found from 0.5 mg · L -1 dauricine without growth inhibition 2.1 mg · L -1 reduced to 1.3 mg · L -1, and 1 mg · L -1 Dau without inhibition inhibited the proliferation of human laryngeal carcinoma HEP2 cells by bleomycin A 5 alone The value of I_ (C50) decreased from 0.33 mg · L -1 to 0.1 mg · L -1 .The results of colony formation assay showed that the non-cytotoxic 10 mg · L -1 Dau significantly enhanced Streptomycin A_5 on HEP2 cytotoxicity TFP method confirmed that a certain growth inhibitory effect of Dau on HEP2 intracellular activated calmodulin results showed that: DHA has a certain degree of enhancement of bleomycin A_5 on tumor cells toxicity.