通过将牛奶中的αs1-酪蛋白、αs2-酪蛋白、β-酪蛋白、κ-酪蛋白、α-乳清蛋白、β-乳球蛋白及白蛋白共7种主要蛋白基因在大肠杆菌中进行了异源表达,并对其中的三种乳清蛋白进行了Western-blot验证。研究结果显示,大肠杆菌可成功表达7种牛奶蛋白,且未被降解。这表明将大肠杆菌作为产生重组牛奶蛋白的底盘细胞具有较好的应用潜能,以上研究对后续进一步开发人造牛奶奠定了基础。 Seven major protein genes of αs1-casein, αs2-casein, β-casein, κ-casein, α-lactalbumin, β-lactoglobulin and albumin in milk were subjected to Escherichia coli Heterologous expression, and three of the whey protein Western-blot validation. The results show that E. coli can successfully express seven kinds of milk protein, and has not been degraded. This indicates that Escherichia coli as a recombinant milk protein production of chassis cells have better potential applications, the above study on the subsequent further development of artificial milk laid the foundation.