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旨在研究白藜芦醇(Resveratrol,RES)对高脂诱导大鼠的生长、血清生化指标、抗氧化以及肠道形态结构的影响。选取6周龄的雄性SD大鼠25只,将大鼠随机分为5组,每组5个重复,每个重复1只。分别饲喂基础饲粮、基础饲粮+低剂量RES(30mg·(kg BW)~(-1))、高脂饲粮、高脂饲粮+低剂量RES(30mg·(kg BW)~(-1))和高脂饲粮+高剂量RES(60mg·(kg BW)~(-1)),试验期4周。结果表明:1)与基础饲粮组相比,高脂饲粮组大鼠体重显著增加(P<0.05),而基础饲粮+低剂量RES组大鼠体重未发生显著变化(P>0.05);与高脂饲粮组相比,高脂饲粮添加低或高剂量白藜芦醇均降低了大鼠体重,但未达到显著水平(P>0.05),而在高脂饲粮中添加低剂量白藜芦醇显著降低了大鼠采食量(P<0.05)。2)与基础饲粮组相比,高脂饲粮组大鼠血清中尿素氮水平和脂肪酸合成酶(FAS)活性显著降低(P<0.05);与高脂饲粮组相比,高脂饲粮添加高剂量白藜芦醇均显著降低大鼠血清中甘油三酯(TG)、非酯化脂肪酸(NEFA)含量(P<0.05),显著升高了高密度脂蛋白(HDL)的含量(P<0.05)。3)在基础饲粮中添加低剂量白藜芦醇显著降低了大鼠血清中GSH-Px的活力(P<0.05);与高脂饲粮组相比,高脂饲粮添加低或高剂量白藜芦醇均能显著降低大鼠血清MDA浓度(P<0.05),提高T-SOD活性及T-AOC(P<0.05)。4)与高脂饲粮组相比,高脂饲粮添加低或高剂量白藜芦醇显著提高了大鼠空肠隐窝深度(P<0.05),且高脂饲粮添加高剂量白藜芦醇有提高绒毛高度的趋势(P<0.1)。综上表明,白藜芦醇可以有效缓解高脂饲粮导致的氧化损伤,促进大鼠生长及其对饲粮养分的利用,改善血脂代谢,提高机体的抗氧化力,但对肠道形态结构未表现出明显的修复效果。
The aim of this study was to investigate the effects of resveratrol (RES) on the growth, serum biochemical parameters, antioxidation and intestinal morphology in rats induced by high fat diet. Twenty-five male SD rats of 6 weeks old were selected and randomly divided into 5 groups with 5 replicates in each group and 1 replicate in each group. The basal diet, basal diet + low dose RES (30 mg · kg -1), high fat diet, high fat diet + low dose RES (30 mg · kg -1) -1)) and high fat diet + high dose RES (60 mg · (kg BW) -1) for 4 weeks. The results showed that: 1) Compared with the basal diet group, the body weight of rats in high fat diet group increased significantly (P <0.05), while the basal diet + low dose RES group had no significant changes in body weight (P> 0.05) Compared with high-fat diet group, high fat diet group fed with low or high doses of resveratrol reduced body weight, but did not reach significant level (P> 0.05), while high fat diet group added low The dose of resveratrol significantly reduced the feed intake of rats (P <0.05). 2) Compared with the basal diet group, the level of serum urea nitrogen and the activity of fatty acid synthase (FAS) were significantly decreased in the high-fat diet group (P <0.05). Compared with the high-fat diet group, High doses of dietary resveratrol significantly reduced the content of triglyceride (TG) and non-esterified fatty acid (NEFA) in serum (P <0.05), and significantly increased the content of high density lipoprotein (HDL) P <0.05). 3) The addition of low dose resveratrol to basal diet significantly reduced the GSH-Px activity in serum of rats (P <0.05). Compared with high-fat diet group, high-fat diet added low or high dose Resveratrol could significantly reduce serum MDA concentration (P <0.05), increase T-SOD activity and T-AOC (P <0.05). 4) Compared with the high-fat diet group, the addition of low or high-dose resveratrol to high-fat diet significantly increased the depth of jejunal crypt in rats (P <0.05), and high-fat diet supplemented with high-dose of white vetch Alcohol tends to increase villus height (P <0.1). In summary, resveratrol can effectively alleviate the oxidative damage caused by high-fat diets, promote the growth of rats and their utilization of dietary nutrients, improve blood lipid metabolism, improve the body’s antioxidant power, but the intestinal morphology Did not show a significant repair effect.