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本研究用甲基硝基亚硝(MNNG)单独处理猴肾细胞后移去残余的诱变剂,然后将穿梭质粒pZ189(含有靶基因supFtRNA基因)引入细胞中复制,并在大肠杆菌MBM7070中筛选突变子。结果显示:0.2μmol/L MNNG处理组的突变频率较自发突变频率增加了3.1倍,而0.2和2μmol/L MNNG处理组点突变频率分别为12.2×10~(-4)(43/35376)和6.2×10~(-4)(22/35712),比自发点突变频率2.1×10~(-4)(10/47741)分别增加了5.8倍和2.9倍,其卡方检验的P值均小于0.01。本研究首次证实了哺乳类细胞中存在着非定标性实变。
In this study, monkey kidney cells were treated with methyl nitro-nitrite (MNNG) alone, and residual mutagen was removed. The shuttle plasmid pZ189 (containing the target gene supFtRNA gene) was then introduced into cells and screened in E. coli MBM7070. Mutants. The results showed that the mutation frequency of the 0.2μmol/L MNNG treated group increased 3.1 times compared with the spontaneous mutation frequency, while the point mutation frequencies of the 0.2 and 2μmol/L MNNG treated group were 12.2×10 -4 (43/35376) and 6.2×10 -4 (22/35712), which was 5.8 times and 2.9 times higher than the spontaneous point mutation frequency of 2.1×10 -4 (10/47741), and the P value of the chi-square test was less than 0.01. This study demonstrated for the first time that there are non-standardized consolidations in mammalian cells.