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为探讨应用昆虫病原线虫防治小地老虎技术的可行性,采用培养皿滤纸法比较9个品系的昆虫病原线虫对小地老虎幼虫的致病力,同时通过盆栽试验验证了斯氏线虫属小卷蛾斯氏线虫Steinernema carpocapsae NC116品系、芫菁夜蛾斯氏线虫S.feltiae SF-SN品系和异小杆线虫属嗜菌异小杆线虫Heterorhabditis bacteriphora H06品系的保苗效果和防治效果,并测定了苦参碱与NC116品系混用对小地老虎3龄幼虫的联合作用效果。结果表明,NC116品系对小地老虎3龄幼虫致病力最高,其对3龄、4龄和5龄的LD50分别为2.4、7.5、和31.2条/头,表明随小地老虎幼虫龄期递增,其致病力下降。盆栽玉米苗上分别施用线虫100~200条/头时,对玉米保苗效果及对小地老虎3龄幼虫的控制效果依次为NC116>H06>SF-SN。0.8 mg/L苦参碱和NC116品系混用后,可使小地老虎3龄幼虫死亡率提高109.89%,二者表现增效作用。
In order to explore the feasibility of using the insect pathogenic nematode to control the small tiger technology, the pathogenicity of the insect pathogenic nematodes of 9 strains to the small-larvae larvae was compared by the culture dish filter paper method. At the same time, The effect of preserving seedlings and controlling effect of Steinernema carpocapsae NC116 strain, S.feltiae SF-SN strain of Heterorhabditis bacteriphora H06 strain and the heterotrophic strain of Heterorhabditis bacteriphora H06 strain were determined, Combined effect of rhizome and NC116 strain on the 3rd instar larvae of. The results showed that strain NC116 had the highest pathogenicity to 3rd instar larvae of Landrace, with LD50 values of 2.4, 7.5, and 31.2 per head for 3rd, 4th, and 5th instars, respectively, indicating an increasing LD50 , Its pathogenicity decreased. When the nematodes were applied on potted maize seedlings in the range of 100-200 per head, respectively, the control effect on the seedling conservation of maize and the 3rd instar larvae of Landrace were NC116> H06> SF-SN. When 0.8 mg / L matrine and NC116 were used in combination, the mortality of 3rd instar larvae of Platycodon regia increased by 109.89%, showing synergism.