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目的测定转基因C57BL/6-Tg(ACTB-EGFP)1Osb/J小鼠血液生理生化正常值。方法采用全自动生化测定仪对5～6周雄性、6～7周雌性EGFP小鼠的血液常规及生化值进行测定。结果EGFP转基因雄雌小鼠血常规比较,雌雄鼠的血红蛋白(Hb)、血小板(PLT)之间比较差异非常显著(P<0.01),单核细胞计数(MO)、红细胞宽度(RDW)之间比较差异显著(P<0.05);血液生化值雄雌小鼠比较,白蛋白(Alb)、甘油三酯(TG)差异非常显著(P<0.01),其它指标差异不显著。结论该小鼠的生理生化指标值在雄雌间有一定的差异,但该值均在正常波动范围。
Objective To determine the normal physiological and biochemical parameters in transgenic C57BL / 6-Tg (ACTB-EGFP) 1Osb / J mice. Methods The blood routine and biochemical values of male and 6 to 7-week-old female EGFP mice aged 5-6 weeks were determined by automatic biochemical analyzer. Results Compared with the blood routine of EGFP transgenic male and female mice, the hemoglobin (Hb) and platelet (PLT) of the male and female rats were significantly different (P <0.01), monocyte count (MO) and red blood cell width (P <0.05). Compared with the blood biochemical values in male and female mice, the differences of albumin (Alb) and triglyceride (TG) were significant (P <0.01), while the other indexes were not significantly different. Conclusion The physiological and biochemical indexes of the mice have some differences between male and female, but the values are in the range of normal fluctuation.