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为了研究氯硝柳胺在斑点叉尾(Ictalurus punctalus)组织中的残留消除规律,在静水条件下经安全性试验确定安全浓度后,直接对水族箱泼洒25%氯硝柳胺乙醇胺盐(NES)粉剂后采集斑点叉尾皮肤、肌肉和水样。采用高效液相色谱-质谱联用法(HPLC-ESI/MS/MS)测定样品中氯硝柳胺的浓度,结果表明,静水条件下25%氯硝柳胺乙醇胺对斑点叉尾的安全浓度为0.113 mg/kg;养殖水环境中氯硝柳胺能快速消解,实验第2天即低于1μg/L,并呈无规律性持续较长时间;用药24 h内,氯硝柳胺在鱼体肌肉和鱼皮中具有较强的蓄积能力,但48 h后,其在鱼皮和肌肉中呈现快速消除至较低浓度,但后期消除缓慢,并会持续一定时间;氯硝柳胺(NIC)在斑点叉尾肌肉和鱼皮中的消除半衰期T1/2分别为10.66和9.24 d,显著大于在水体中的消除半衷期。
In order to study the rule of residual elimination of niclosamide in Ictalurus punctalus tissue, 25% niclosamide ethanolamine salt (NES) was directly splashed into the aquarium after the safe concentration was determined by safety test in still water. After the powder was collected channel catfish skin, muscle and water samples. The concentration of niclosamide in the samples was determined by HPLC-MS / MS. The results showed that the concentration of niclosamide ethanolamine 25% in the water was 0.113 mg / kg; niclosamide in aquaculture water can quickly digestion, the first two days of the experiment is less than 1μg / L, and irregular for a long time; medication within 24 h, niclosamide in fish muscle And fish skin had strong accumulation ability, but after 48 h, it showed rapid elimination in fish skin and muscle to a lower concentration, but the late elimination was slow and continued for a certain period of time. Niclosamide (NIC) The elimination half-life T1 / 2 in channel catfish muscle and fish skin was 10.66 and 9.24 d, respectively, which was significantly greater than that in water body.