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2009107 Comparison of the clinical application of different methods for detection of NPM1 gene mutations in leukemia. ZOU Jiyan(邹积艳),et al.Dept Hematol, 1st Hosp, Peking Univ, Beijing 100034. Chin J Lab Med 2009;32(1):35-39. Objective To analyze nucleophosmin (NPM1) gene mutations in exon 12 in patients with acute myeloid leukemia (AML) and evaluate the clinical appliance of three methods which are frequently used for detecting gene mutation. Methods Genomic DNA from bone marrow of 54 AML patients was detected by PCR for NPM1 exon 12 and screened by PCR-capillary electrophoresis, denature high performance liquid chromatography (DHPLC) and direct sequencing separately. FLT3-ITD (FMS-like tyrosine kinease internal tandem duplication) was detected by agarose gel electrophoresis and PCR-capillary electrophoresis. Results Seven AML sample harbored NPM1 gene mutations.
2009107 Comparison of the clinical application of different methods for detection of NPM1 gene mutations in leukemia. ZOU Jiyan, et al. Dept Hematol, 1st Hosp, Peking Univ, Beijing 100034. Chin J Lab Med 2009; 32 (1) 35-39. Objective To analyze nucleophosmin (NPM1) gene mutations in exon 12 in patients with acute myeloid leukemia (AML) and evaluate the clinical appliance of three methods which are frequently used for detecting gene mutation. Methods Genomic DNA from bone marrow of 54 AML patients was detected by PCR for NPM1 exon 12 and screened by PCR-capillary electrophoresis, denature high performance liquid chromatography (DHPLC) and direct sequencing separately. FLT3-ITD (FMS-like tyrosine kinease internal tandem duplication) was detected by agarose gel electrophoresis Results Seven AML sample harbored NPM1 gene mutations.