采用ＲＴＰＣＲ方法从中国人肾癌细胞株中克隆到甲状旁腺素相关蛋白（Ｐａｒａｔｈｙｒｏｉｄｈｏｒｍｎｏｅｒｅｌａｔｅｄｐｒｏｔｅｉｎ，ＰＴＨｒＰ）ｃＤＮＡ，其核苷酸序列与国外发表资料相比，有６个核苷酸不同，其中仅９２位密码子的不同导致氨基酸变异，即由脯氨酸变为丝氨酸。将克隆的ＰＴＨｒＰｃＤＮＡ插到原核表达载体ｐＥＴ３ａ的Ｔ７噬菌体启动子下游，转化大肠杆菌后得到高效表达，并经Ｗｅｓｔｅｒｎｂｌｏｔ和生物学活性检测对表达产物作了鉴定。 The cDNA of parathyroid hormone related protein (PTHrP) was cloned from Chinese human renal cell carcinoma cell line by RTPCR. The nucleotide sequence of the cDNA was 6 nucleotides different from that published in foreign countries. The difference in codon 92 results in a variation in amino acids, from proline to serine. The cloned PTHrPcDNA was inserted into the prokaryotic expression vector pET  3a downstream of the T7 phage promoter, transformed into E. coli after high expression, and the expression product was identified by Western blot and biological activity test.