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目的比较体外培养的正常乳腺细胞和乳腺癌细胞核蛋白指纹图谱,筛选差异蛋白,为乳腺癌亚细胞蛋白标志物的研究奠定基础。方法提取正常乳腺细胞株HBL-100和乳腺癌细胞株MCF-7、MDA-MB-231的核蛋白,表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)检测蛋白表达,重复试验30次,Biomaker Wizard Software分析软件统计结果并筛选差异蛋白。结果分子量(Mr)2～100kD范围内MCF-7和MDA-MB-231与HBL-100相比差异蛋白点数目分别为78个和69个(P﹤0.05),其中5.8kD、8.3kD和18.2kD的蛋白在两株癌细胞中表达均降低,9.9kD和15.7kD的蛋白在两株癌细胞中表达均增高。结论 SELDI-TOF-MS能够快速灵敏地检测分析乳腺癌细胞核差异表达蛋白,为乳腺癌亚细胞蛋白标志物的研究提供了新思路。
Objective To compare the fingerprints of normal breast cells and breast cancer cells cultured in vitro and screen the differential proteins to lay a foundation for the study of breast cancer subcellular protein markers. Methods The protein levels of HBL-100, MCF-7 and MDA-MB-231 were detected by surface-enhanced laser desorption / ionization time of flight mass spectrometry (SELDI-TOF- , Biomaker Wizard Software analyzes software statistics and screens differential proteins. Results Compared with HBL-100, the number of differentially expressed protein spots in MCF-7 and MDA-MB-231 ranged from 78 to 69 (P <0.05) in the range of 2 to 100 kD Mr, 5.8 kD, 8.3 kD and 18.2 The expression of kD protein in both cancer cells decreased, and the expression of 9.9kD and 15.7kD protein increased in both cancer cells. Conclusion SELDI-TOF-MS can detect and analyze the differentially expressed proteins in breast cancer cells rapidly and sensitively, which provides a new idea for the study of breast cancer subcellular protein markers.