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采用MTT和SRB法研究了混胺(NH3/CH3NH2)草酸根合铂(Ⅱ)混配配合物(MAOX)对人体癌细胞的增殖抑制作用,又通过流式细胞法、Giemsa染色法、等离子体质谱法研究了它的抗癌机制.实验结果表明:在10μmol.L-1浓度下,该配合物对HL-60和EJ两种细胞有较高的活性,其抑制率分别为51.35%和45.86%;它能阻止HL-60细胞G2+M→G1期的进行,造成G2+M期细胞的堆积;对HL-60细胞的凋亡诱导作用不明显;在相同浓度情况下其与HL-60细胞的DNA键合量大于顺铂.
MTT and SRB were used to study the inhibitory effect of mixed NH3 / CH3NH2 oxalate (MAOX) on the proliferation of human cancer cells. The effects of NH3 / CH3NH2 on the proliferation of human cancer cells were also studied by flow cytometry, Giemsa staining, Its mechanism of anticancer activity was studied by mass spectrometry.The experimental results showed that the complex had higher activity on both HL-60 and EJ cells at a concentration of 10μmol.L-1 with the inhibitory rates of 51.35% and 45.86 %; It can prevent the G2 + M → G1 phase of HL-60 cells from progressing, resulting in the accumulation of G2 + M phase cells; The apoptosis induction effect on HL-60 cells is not obvious; Under the same concentration, Cell DNA binding is greater than cisplatin.