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目的研究纳米铜的肾细胞毒性,探讨纳米铜的溶出度在纳米铜致大鼠肾毒性中的作用。方法利用MTT法和LDH漏出率测定纳米铜的细胞毒性,利用一价铜特异性螯合剂、分光光度法、电感耦合等离子体-原子发射光谱法分析纳米铜粒子在培养体系中的溶出度。结果纳米铜可以剂量依赖性方式诱导细胞活力的下降,作用24 h对HK-2细胞的IC50为41.3μg.mL-1;纳米铜可以剂量依赖性方式引起细胞的LDH漏出增加,一价铜离子特异性螯合剂BCS可抑制纳米铜或氯化铜诱导的细胞毒性;在培养体系中,纳米铜粒子的溶解率呈现时间依赖性升高。结论纳米铜对肾细胞具有明显毒性作用;纳米铜在一定剂量范围内,转化为铜离子是其发挥细胞毒作用的重要方式。
Objective To study the renal cell toxicity of nano-copper and explore the role of the dissolution of nano-copper in renal toxicity induced by nano-copper in rats. Methods MTT assay and LDH leakage rate were used to determine the cytotoxicity of nano-copper. The dissolution of nano-copper particles in culture system was analyzed by monovalent copper-specific chelating agent, spectrophotometry and inductively coupled plasma atomic emission spectrometry. Results Nano-copper induced a decrease of cell viability in a dose-dependent manner. IC50 of HK-2 cells for 24 h was 41.3μg.mL-1. Nano-copper induced LDH leakage in a dose-dependent manner. Monovalent copper ions The specific chelator BCS could inhibit the cytotoxicity induced by nano-copper or copper chloride. In the culture system, the dissolution rate of nano-copper particles showed a time-dependent increase. Conclusion Nano-copper has a significant toxic effect on renal cells. The conversion of copper nanoparticles to copper ion is an important way of its cytotoxicity in a certain dosage range.