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目的 把握口咽部非厌氧正常菌群的携带率和量 ,为准确识别呼吸道感染菌奠定基础。方法 随机采取不同年龄段健康人群口咽拭子 ,分区划线于血、巧克力、TTC沙保罗平板 ;巧克力平板置烛缸 ,其余置空气环境 ,35℃孵育 48小时 ,分别挑取各种菌落并于第 1区混合取材涂片 ,革兰染色镜检 ,必要者加作触酶、凝固酶等相关试验 ;TTC沙保罗平板培养 1周 ;于血和巧克力平板计数 10 0个菌落 ,用十分法记录各种细菌的数量。结果 口咽部非厌氧正常菌群以非β-溶血链 (肠 )球菌和革兰氏阴性球菌居主 ,携带率均 10 0 % ,平均携带量前者 0 .45 ,后者 0 .35 ;微球菌和棒状杆菌居次 ,携带率分别为 74%和 6 7% ,平均携带量均 <0 .1;革兰阴性杆菌、凝固酶阴性葡萄球菌、肺炎链球菌和深部真菌较少 ,其携带率分别为 33%、2 8%、7.5 %和 4.7% ,平均携带量均 <0 .1。结论 若某种细菌数量明显增多 ,则有成为感染菌的可能。
OBJECTIVE To grasp the carrying rate and quantity of normal anaerobic oropharyngeal non-anaerobic bacteria and lay the foundation for accurate identification of respiratory tract infections. Methods Randomly take oropharyngeal swabs of healthy people of different ages and subdivide them into blood, chocolate and TTC sand paulownia plate; chocolate plate was placed in the candle jar and the rest set in air environment and incubated for 48 hours at 35 ℃, Mixed smear in the first area, Gram stain microscopy, if necessary, plus for catalase, coagulase and other related tests; TTC sand Paul slab culture for 1 week; in blood and chocolate plates count 10 0 colonies, by ten method Record the number of various bacteria. Results The non-anaerobic normal population of oropharynx was predominantly non-β-hemolytic chain (Enterococcus) and Gram-negative cocci, carrying rate was 100%, the average carrying capacity of the former 0.45, the latter 0.35; Micrococci and Corynebacterium were the second most frequent carriers with rates of 74% and 67%, respectively, with an average carrying capacity of <0.1. Gram-negative bacilli, coagulase-negative staphylococci, S. pneumoniae and deep fungi carried less The rates were 33%, 28%, 7.5% and 4.7%, respectively, with an average carrying amount of <0.1. Conclusion If a significant increase in the number of bacteria, there is the possibility of becoming infected bacteria.