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根据NPTⅡ基因 (卡那霉素标记基因 )与Bt抗虫基因紧密连锁的关系 ,试验研究了利用卡那霉素水溶液点涂叶片在田间快速鉴定携带转Bt基因抗虫棉株的方法。虫测鉴和Bt基因遗传规律调查证明该方法简便、快速、有效 ,可在转基因抗虫棉育种选择和繁种保纯中应用。
According to the close linkage between the NPTⅡgene (kanamycin marker gene) and Bt insect-resistant gene, a rapid method for the identification of insect-resistant cotton plants carrying the Bt gene in the field was carried out by spot coating the leaves with kanamycin solution. The survey of worm and Bt gene showed that the method was simple, rapid and effective, and could be used in selection and breeding of transgenic Bt cotton.