营养与品种对肉牛血清中AMPK活性与血液生化指标关系的研究

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本研究旨在探讨营养与品种对肉牛血清中磷酸腺苷活化蛋白激酶( AMPK)活性的影响及AMPK活性与血液生化指标的关系.选取体重为250 kg左右的西杂牛35头、利杂牛26头、夏杂牛24头,每个品种牛设低、中、高3个日粮营养水平,育肥期为10个月.试验结束后采集肉牛血液,分析营养与品种对血清中AMPK活性的影响,同时探讨AMPK活性与脂肪代谢相关生化指标的关系.结果表明:营养与品种对血液中各生化指标都不存在显著互作效应.从营养上看,高营养时AMPK活性和高密度脂蛋白(HDL)含量较低,乙酰辅酶A羧化酶(ACC)活性和甘油三酯(TG)、胆固醇(TC)含量都随着营养的提高而增加;从品种上看,利杂牛AMPK、ACC活性和HDL含量较高,夏杂牛TG、TC含量较高;营养与品种对HMGR活性和葡萄糖(GLU)含量都没有显著影响.AMPK活性与HDL含量呈正相关,与ACC、HMGR活性和TG、TC、GLU含量呈负相关.可见,高营养会降低AMPK活性,而品种差异也影响AMPK活性,AMPK活性与脂肪代谢密切相关,影响肉牛脂肪沉积.
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