Brucellosis,an allergy anthropozoonosis caused by Brucella,is widely prevalent all over the world [1].Its incidence is increasing in several endemic areas and it has great impact on human and animal health [2].Accurate diagnosis of human brucellosis is difficult,which often causes omission or mistake in patients.Many methods have been used for serological diagnosis of brucellosis.However,a single ideal technique is currently unavailable [3].Among these methods,the rose bengal plate agglutination test (RBPT) [4] is excellent with simple,rapid,and sensitive characteristics irrespective of the stage of the disease.The standard tube agglutination test (SAT) [5] is another most widely used serological test for confirmation of RBPT-positive serum.However,it suffers from a high false-negative ratio in complicated and chronic cases [3].Brucella enzyme-linked immunosorbent assay (ELISA;IgM,IgG) is the confirmatory test for diagnosis of chronic disease and relapse [6].The accuracy of these methods is ranked as ELISA ＞ RBPT ＞ SAT [7].Sisirak et al.[3] found that blood culture leads to excellent results in 30％ patients with primary infection especially in the first month.Although it is the ‘gold standard for diagnosis of brucellosis,this technique needs many expensive laboratory setups,which blocks its use in an underdeveloped area with poor technical resources.