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本文应用表面增强技术将薄层色谱与近红外傅立叶变换拉曼光谱联用 ,获得到了中草药萝芙木生物碱中主要有效成分育亨宾在薄层原位的傅立叶变换表面增强拉曼光谱 .研究表明 ,在薄层色谱直径约 2mm斑点原位 ,样品的FT -SERS光谱与纯固体样品的FT -Raman光谱的主要特征峰波数基本一致 ,相对强度出现一些变化 ,固体与薄层色谱原位谱图中 ,指纹区最强峰都是表征吲哚环骨架伸缩振动、波数为 15 90cm- 1 附近的峰 .育亨宾分子以吲哚环共轭体系的π电子与表面增强活性物质银晶体微粒表面相互作用引起拉曼散射的显著增强 .获得了中草药萝芙木中主要有效成分育亨宾分离与高灵敏度指纹检测可靠的新方法 .
In this paper, the surface enhancement technique was used to combine thin-layer chromatography with near-infrared Fourier transform Raman spectroscopy to obtain the Fourier transform surface enhanced Raman spectra of yohimbin, the major active component of the Chinese herbal medicine Rauwolfia alkaloids, in the thin layer. The results show that the FT-SSS spectrum of the sample is almost the same as that of the FT-Raman spectrum of the pure solid sample, and the relative intensity shows some changes. The in-situ spectra of the solid and thin layer chromatography In the figure, the strongest peak in the fingerprint region is the peak that characterizes the stretching vibration of the indole ring skeleton with a wave number of around 1590 cm-1. Yohimbe molecules take the π-electron of the indole ring conjugate system and the surface-enhanced active substance silver crystal particles Surface interaction caused a significant increase in Raman scattering obtained a new method of yohimbine separation and sensitive fingerprint detection of the main active ingredient in Chinese herbal medicine Rauvolfia.