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目的建立一种快速、特异的检测大肠杆菌O157:H7毒力的多重PCR方法。方法选用针对大扬杆菌O157:H7志贺样毒素Ⅰ、Ⅱ的两对引物,在同一扩增体系中进行PCR,然后进行琼脂糖电泳,检测不同来源的大肠杆菌O157:H7及E.coli-ATCC25922株。结果EHEC O157:H7933W株扩增出结果均为阴性。结论多重PCR方法检测大肠杆菌O157:H7毒力基因,具有简便、快速、特异、敏感等特点,对流行病学调查分析、制定预防和控制对策有重要的参考价值。
Objective To establish a rapid and specific multiplex PCR method for detecting the virulence of E. coli O157: H7. Methods Two pairs of primers targeting Shigella O157: H7 shiga-like toxin I and II were selected and PCR was performed in the same amplification system. The agarose gel electrophoresis was performed to detect E. coli O157: H7 and E. coli- ATCC25922 strain. Results The results of EHEC O157: H7933W strain were negative. Conclusion The multiplex PCR method for detecting virulence genes of E. coli O157: H7 is simple, rapid, specific and sensitive. It has important reference value for epidemiological investigation and prevention and control measures.