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单纯疱疹病毒(HSVⅠ)对宿主细胞转录及mRNA修饰翻译通路的调控是一个系统的、复杂的体系,探索其具体机制将有助于了解病毒复制过程及与宿主细胞之间的相互作用。基于2-DE分析,人正常肝细胞L-02细胞核不均一性核糖核蛋白H2(heterogeneous nuclear ribonucleoprotein H2,hnRNP H2)在感染HSVⅠ前后存在表达差异,通过Western blot,Northern blot等技术方法验证其在病毒复制过程中不同时段对其表达影响。
The regulation of herpes simplex virus (HSVⅠ) on the host cell transcription and mRNA translation pathway is a systematic and complex system. Exploring its specific mechanism will help to understand the process of virus replication and its interaction with host cells. Based on 2-DE analysis, the difference of expression of heterogeneous nuclear ribonucleoprotein H2 (HnRNP H2) in human normal liver cells before and after infection with HSVⅠwas confirmed by Western blot and Northern blot The virus replication in different periods of its expression.