目的建立高效液相色谱法测定维生素B1片含量均匀度。方法采用色谱柱:Diamonsil C18(150mm×4.6mm,5μm),以碳十八烷基键合硅胶为填充剂。流动相:甲醇-乙腈-0.02mol/L庚烷磺酸钠溶液(含1%三乙胺,用磷酸调节pH值至5.5,9∶9∶82);检测波长为238nm,流速为1.0ml/min;进样量:10μl。结果维生素B1质量浓度在50.5~505μg/ml范围内与峰面积呈良好的线性关系(r=0.99999,n=5),平均回收率为100.4%,RSD=0.13%。结论本方法简单、精确、可靠,可作为维生素B1片的质量控制方法。 Objective To establish a HPLC method for the determination of the content of vitamin B1 tablets. Methods Column: Diamonsil C18 (150mm × 4.6mm, 5μm), with carbon octadecyl bonded silica as a filler. Mobile phase: methanol-acetonitrile-0.02mol / L sodium heptane sulfonate solution (containing 1% triethylamine, pH adjusted to pH5.5,9: 9:82 with phosphoric acid); the detection wavelength was at 238nm and the flow rate was 1.0ml / min; injection volume: 10μl. Results The concentration of vitamin B1 showed a good linear relationship with the peak area in the range of 50.5 ~ 505μg / ml (r = 0.99999, n = 5). The average recovery was 100.4% and RSD was 0.13%. Conclusion This method is simple, accurate and reliable and can be used as a quality control method for vitamin B1 tablets.