目的:研究三叶青黄酮对荷Lewis肺癌模型小鼠脾脏Treg细胞生成及其CD152表达的影响。方法:将小鼠随机分为非造模组、生理盐水组、塞来昔布组、三叶青黄酮高、中、低剂量组,除非造模组外,其它各组皮下接种法建立Lewis肺癌小鼠模型,接种24 h后开始灌胃给药,每天1次,非造模组不灌胃。分别于给药14、23天后,处死半数动物,取脾脏,分离单个核细胞,流式细胞术测定脾脏单个核细胞中Treg细胞比例及CD152表达。结果:第23天时,与生理盐水组比较,塞来昔布组、三叶青黄酮高、中剂量组小鼠的瘤体明显减小,差异均有统计学意义(P<0.01)。三叶青黄酮各剂量组间瘤体比较存在差异(P<0.05),瘤体大小依次为:三叶青黄酮高剂量组<三叶青黄酮中剂量组<三叶青黄酮低剂量组;塞来昔布组抑瘤效果与中剂量组相近(P>0.05)。与非造模组比较,其它各组小鼠Treg细胞比例和CD152的表达均明显升高,差异均有统计学意义(P<0.01)。第23天时,与生理盐水组比较,各给药组小鼠Treg细胞比例和CD152的表达均明显降低,差异均有统计学意义(P<0.05,P<0.01)。三叶青黄酮各剂量组间Treg细胞比例比较存在差异(P<0.01),Treg细胞比例依次为:三叶青黄酮高剂量组<三叶青黄酮中剂量组<三叶青黄酮低剂量组;塞来昔布组Treg比例与中剂量组相近(P>0.05)。塞来昔布组下调CD152表达效果最佳,与三叶青黄酮中、低剂量组比较有显著差异(P<0.05),三叶青黄酮各剂量组间无明显差异(P>0.05)。结论:三叶青黄酮的抑瘤效果可能与下调Treg细胞比例和CD152表达有关,逆转免疫抑制,从而起到抗肿瘤的作用 Objective: To study the effects of cloverhye flavonoids on the production of Treg cells and the expression of CD152 in spleen of Lewis lung carcinoma model mice. Methods: The mice were randomly divided into non-model group, saline group, celecoxib group, clover flavonoids high, medium and low dose groups, except for the model group, the other groups were subcutaneously vaccinated to establish Lewis lung cancer Mouse model, gavage 24 h after inoculation, once a day, non-model group did not gavage. Fifty-three and thirty-three days after the administration, half of the animals were killed, the spleen was taken and mononuclear cells were isolated. The proportion of Treg cells and the expression of CD152 in spleen mononuclear cells were measured by flow cytometry. Results: On the 23rd day, compared with the saline group, the tumor volume of celecoxib group and clovermectin high and medium dose groups were significantly reduced, the difference was statistically significant (P <0.01). The results showed that there were significant differences in the tumor size between clover leaf flavonoids (P <0.05) and tumor size in the order: high dose clovermectin group 0.05). Compared with the non-model group, the percentage of Treg cells and the expression of CD152 in the other groups were significantly increased, the difference was statistically significant (P <0.01). On the 23rd day, the percentage of Treg cells and the expression of CD152 in each group were significantly decreased compared with those in the saline group, with statistical significance (P <0.05, P <0.01). There was significant difference in the proportion of Treg cells between each dose group of clover leaf flavonoids (P <0.01). The proportion of Treg cells in order was: high dose clovermectin group 0.05). The expression of CD152 in celecoxib group was the best, with significant difference (P <0.05) compared with the middle and low doses of clover leaf flavonoids, and there was no significant difference between each dose of clover leaf flavonoids (P> 0.05). CONCLUSION: The antitumor effect of cloverhye flavonoids may be related to the downregulation of Treg cells and the expression of CD152, reversing the immunosuppression and thus exerting anti-tumor effect