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为探讨单纯疱疹病毒性脑炎(HSVE)的诊断,应用间接ELISA法测定70例病毒性脑炎患儿脑脊液的IgM、IgG,同时对15例患儿的血清进行HSV特异性IgG的检测;采用ELISA双抗夹心法对70例患儿的脑脊液同时检测HSV抗原。结果:脑脊液HSV抗原阳性20例,IgM阳性8例,IgG血清/脑脊液比值≤20为12例。对HSVE患儿予以连续静脉滴注无环鸟苷7天,结果治愈、好转20例(80％)。提示应用ELISA法测定脑脊液HSV特异性抗原和IgM以及IgG血清/脑脊液比值,结合临床可早期诊断HSVE,对HSVE的治疗及改善预后有指导意义。
To investigate the diagnosis of herpes simplex virus encephalitis (HSVE), IgM and IgG in cerebrospinal fluid of 70 children with viral encephalitis were detected by indirect ELISA. HSV specific IgG was detected in serum of 15 children. ELISA double antibody sandwich method in 70 cases of children with cerebrospinal fluid simultaneous detection of HSV antigen. Results: Cerebrospinal fluid HSV antigen positive in 20 cases, IgM positive in 8 cases, IgG serum / CSF ratio ≤ 20 in 12 cases. HSVE children given continuous intravenous acyclovir 7 days, the results were cured, improved in 20 cases (80%). It is suggested that the ratio of HSV-specific antigen, IgM and IgG serum / cerebrospinal fluid in cerebrospinal fluid can be measured by ELISA. Combined with clinical diagnosis of HSV can be beneficial for the treatment of HSVE and prognosis.