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目的建立同时测定香青兰提取物中1种苯丙素类成分(迷迭香酸)和2种黄酮类成分(木犀草素-7-O-β-D-葡萄糖醛酸苷和田蓟苷)的含量测定方法。方法采用PurospherSTARLP RP-18 endcapped(4.6 mm×250 mm,5μm)色谱柱,流动相为乙睛(A)-0.5%甲酸水溶液(B),梯度洗脱(0~28 min,20%A;28~55 min,20%→28%A;55~80 min,28%→30%A),流速为1.0 m L·min-1,检测波长为330 nm,柱温为35℃。结果迷迭香酸、木犀草素-7-O-β-D-葡萄糖醛酸苷和田蓟苷分别在2.184~21.84μg·m L-1(r=0.999 6),3.14~31.84μg·m L-1(r=0.999 6),7.9~39.5μg·m L-1(r=0.999 5)内呈良好线性关系。香青兰提取物迷迭香酸、木犀草素-7-O-β-D-葡萄糖醛酸苷和田蓟苷的平均回收率(n=9)分别为99.62%、99.64%和100.12%,且RSD分别为1.27%、1.05%和1.09%。结论本方法准确、简便,重复性好。可用于同时测定香青兰提取物中木犀草素-7-O-β-D-葡萄糖醛酸苷、迷迭香酸和田蓟苷的含量。
Objective To establish a method for the simultaneous determination of one phenylpropanoid (rosmarinic acid) and two flavonoids (luteolin-7-O-β-D-glucuronide and thymidine) Determination of content. Methods Purospher STARLP RP-18 endcapped (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of acetonitrile (A) -0.5% formic acid (B) and gradient elution ; 28-55 min, 20% → 28% A; 55-80 min, 28% → 30% A) at a flow rate of 1.0 m L · min-1 with a detection wavelength of 330 nm and a column temperature of 35 ° C. Results Rosmarinic acid, luteolin-7-O-β-D-glucuronide and silymarin were in the range of 2.184-21.84μg · m L -1 (r = 0.999 6), 3.14-31.84μg · m L -1 (r = 0.999 6), 7.9 ~ 39.5 μg · m L -1 (r = 0.999 5). The average recoveries (n = 9) for the Fragrant blueberry extracts of rosmarinic acid, luteolin-7-O-β-D-glucuronide and thymidine were 99.62%, 99.64% and 100.12% RSD were 1.27%, 1.05% and 1.09% respectively. Conclusion This method is accurate, simple and reproducible. It can be used for the simultaneous determination of luteolin-7-O-β-D-glucuronide, rosmarinic acid and thymidine in the extract.