论文部分内容阅读
为了解微量元素硒、锌在动物体内的抗脂质过氧化作用,将40只普通级成年ICR小鼠随机均分为4组:①对照组,饮蒸馏水;②补硒组,饮水中加硒,浓度为0.5mg/L;③补锌组,饮水中加锌,浓度为12.5mg/L;④补硒和锌组,饮水中加硒和锌,浓度分别与第2、3组相同。实验期为5周,饲料为上海西普尔-必凯实验动物有限公司的颗粒饲料,实验最后1天只供饮水,不喂食。然后用经作者改良的硫代巴比妥酸反应法测脑、肝和血清中脂质过氧化物(LPO)含量(以丙二醛的含量表示)。结果,对照组动物脑、肝的LPO水平分别为112.2±7.7、74.3±6.0nmol/g,
In order to understand the anti-lipid peroxidation of trace elements selenium and zinc in animals, 40 common adult ICR mice were randomly divided into four groups: ① control group, drinking distilled water; ② selenium group, selenium in drinking water , The concentration of 0.5mg / L; ③ zinc supplementation group, drinking water plus zinc, the concentration of 12.5mg / L; ④ selenium and zinc supplementation group, add selenium and zinc in drinking water, concentrations were the same with the second and third groups. The experimental period was 5 weeks. The feed was pellet feed of Shanghai Xipu-Kekai Experimental Animal Co., Ltd. The last day of the experiment was only for drinking water and no feeding. The content of lipid peroxides (LPO) in the brain, liver and serum (expressed as malondialdehyde) was then determined by the authors’ modified thiobarbituric acid reaction method. As a result, the LPO levels in the brain and liver of the control animals were 112.2 ± 7.7 and 74.3 ± 6.0 nmol / g, respectively,