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FeCl3为氧化剂合成了聚苯胺纳米纤维,纤维直径20~30 nm,并以此为载体对辣根过氧化物酶进行了固定化.SEM,FTIR,UV-Vis等结构表征证明辣根过氧化物酶HRP中的质子可作为掺杂剂与聚苯胺主链中的氮结合,酸根离子作为对阴离子依附于聚苯胺主链周围,实现了聚苯胺载体对生物酶的固定化.聚苯胺纳米纤维具有较大的比表面积,有利于增加酶的负载量,提高固定化酶活性.该方法简单,快速,载体材料无需活化.与游离酶相比,固定化酶对pH,高温的耐受性,不同温度下的热稳定性均有了明显的提高,在4℃条件下保存7周后固定化酶活性几乎没有损失,重复使用6次后仍可保持80%的酶活,说明纳米结构的聚苯胺是一种良好的载体,可实现生物酶的高效固定化.
FeCl3 was used as oxidant to synthesize polyaniline nanofibers with fiber diameter of 20-30 nm and immobilized on horseradish peroxidase (HRP) .SEM, FTIR and UV-Vis were used to characterize the structure of horseradish peroxidase The proton in the enzyme HRP can be used as a dopant to combine with the nitrogen in the polyaniline backbone, and the acid ions act as the anion to attach to the periphery of the polyaniline backbone, thus immobilizing the immobilized enzyme on the polyaniline carrier. Polyaniline nanofibers have The larger specific surface area is conducive to increasing the loading of the enzyme and increasing the activity of the immobilized enzyme. The method is simple, rapid and requires no activation of the carrier material. Compared with the free enzyme, the immobilized enzyme has different tolerance to pH and high temperature The thermal stability at the temperature has been significantly improved, after storage at 4 ℃ for 7 weeks immobilized enzyme activity almost no loss, after repeated use of 6 can still maintain 80% of the enzyme activity, indicating that nano-structured polyaniline Is a good carrier, enabling efficient immobilization of biological enzymes.