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目的:应用单光子发射计算机断层扫描观察家兔通过皮下、皮内、静脉3种不同途径注射核素钼锝“风门”穴的皮部腧穴通路,分析腧穴与皮肤感受器的关系。方法:实验于2005-10/11在徐州医学院附属连云港医院肿瘤生物实验室完成。将6只家兔分为皮下注射组,皮内注射组和静脉注射组3组,每组2只,皮下注射组在已确定的家兔胃伤害性刺激的皮肤牵涉区,取双侧“风门”穴,左侧皮内、右侧皮下注射核素钼锝(99TcmO4-,99Tcm)为示踪剂,皮内注射组取双侧″风门″穴,双侧皮内注射99Tcm,静脉注射组经静脉注射99Tcm。各组均应用单光子发射计算机断层扫描成像,分别从兔冠状面和矢状面收集示踪剂图像,示踪剂选择绿色成像,示踪剂集中区呈白色。每次连续收集图象16幅,成像时间为7min,开始每隔15min连续收集图像1次,1h后每隔30min连续收集图像1次,3h后每隔60min连续收集图像1次,6h后每隔120min连续收集图像1次,观察至注射核素后12～14h。结果:①实验家兔6只均成功按设计方案进行了示踪剂的注射,3组实验共获单光子发射计算机断层扫描图像1280幅。②皮下、皮内、静脉3种不同途径注射核素钼锝通过SPECT观察不同的形态学变化:“风门”穴皮内注射的99Tcm沿纵向行走,延长约2～5倍,注射12～14h后仍清晰可见;“风门”穴皮下注射的99Tcm逐渐缩小,8～10h消失;静脉注射的99Tcm,8～10h大部分经肾脏排泄。结论:①家兔皮内注药有两条吸收途径,即血液和神经末梢感受器吸收途径。②皮下注射主要通过血液吸收。③腧穴部位皮内示踪剂沿纵向行走,有可能与皮内神经末梢感受器的逆行吸收有关,与交感感受器关系有可能更密切,皮部腧穴有可能是皮内感受器的密集点或片,皮部经络有可能是皮内感受器密集点的有序排列。
OBJECTIVE: To observe the relationship between acupoints and skin receptors in rabbits by injecting subcutaneous, intraepithelial, and intravenous injection of mucolytic technetium “Fengmen” acupoints via single photon emission computerized tomography. METHODS: The experiment was performed at the Tumor Biology Laboratory of Lianyungang Hospital affiliated to Xuzhou Medical College from October to October 2005. Six rabbits were divided into subcutaneous injection group, intradermal injection group and intravenous injection group. Each group had 2 rabbits in each group. In the subcutaneous injection group, “, The left intradermal, the right subcutaneous injection of molybdenum and molybdenum technetium (99TcmO4-, 99Tcm) as a tracer, intradermal injection group to take bilateral” throttle “hole, bilateral intradermal injection of 99Tcm, intravenous group Intravenous injection 99Tcm. Single-photon emission computed tomography imaging was used in each group. Tracer images were collected from the coronal and sagittal planes of the rabbits. Tracer was selected for green imaging and the tracer concentration zone was white. Each successive collection of images 16, the imaging time was 7min, began to collect images every 15min consecutive images 1h after 30min continuous collection of images once every 3h after 60min continuous collection of images 1, 6h after every interval 120min continuous collection of images 1 times observed until after injection of nuclides 12 ~ 14h. Results: ① All the 6 rabbits were successfully injected with tracer according to the design scheme. There were 1,280 single photon emission computed tomography images in 3 groups. Morphological changes of molybdenum and technetium were observed by SPECT in three different ways: subcutaneously, intradermally and intravenously. The 99Tcm of ”Fengmen“ intradermal injection was longitudinally extended by about 2 ~ 5 times, and after 12 ~ 14h injection Still clearly visible; ”throttle" subcutaneous injection of 99Tcm gradually reduced 8 ~ 10h disappeared; intravenous injection of 99Tcm, 8 ~ 10h most of the excretion by the kidneys. Conclusion: ① Rabbit intradermal injection has two absorption ways, that is, blood and nerve endings receptor absorption pathway. ② subcutaneous injection mainly through the blood absorption. ③ acupoints intradermal tracer walking along the longitudinal direction, may be related to the retrograde absorption of nerve endothetes in the dermis, and the sympathetic receptors may be more closely, the skin acupoints may be dense point of the intradermic receptors or tablets, skin Meridian may be an orderly arrangement of dense points of the intradermic receptors.