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报道了利用DNA重组技术在一段对虾白斑杆状病毒 (WhiteSpotBacilliformVirus ,,WSBV)的基因组DNA片段中缺失 74bp ,重组得到的内标质粒作为竞争性内标模板 ,并与阳标模板或病虾DNA模板共同扩增 ,建立了定量PCR检测方法。研究表明 ,定量PCR方法检测灵敏度达 10病毒分子DNA/ μl,样品DNA模板制备回收率约 50 % ,并能对病虾样品中的WSBV含量进行定量分析
Reported the use of recombinant DNA technology in a fragment of shrimp white spot baculovirus (WSBV) genomic DNA fragment missing 74bp, the recombinant plasmid obtained as a competitive internal standard template, and positive or negative template DNA template Co-amplification, established a quantitative PCR detection method. The results showed that the quantitative PCR method could detect 10 DNA molecules / μl, the recovery rate of DNA template was about 50%, and the content of WSBV in the shrimp samples could be quantitatively analyzed