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为获得甜叶菊的最佳增殖培养基以及60 Co-γ辐射诱变育种的剂量,以无菌甜叶菊茎段为外植体,采用正交实验对其增殖培养基的激素种类和浓度进行优化;在此基础上,分别采用5、15、30、60、90、120和150Gy剂量的60Co-γ射线对甜叶菊茎段进行辐照诱变,每隔15d记录其生长情况,统计死亡率;并测定≤30Gy剂量处理的叶片中可溶性多糖和可溶性蛋白质的含量。结果表明,甜叶菊茎段增殖的最佳培养基为MS+NAA 0.5mg·L-1+TDZ0.1mg·L-1+6-BA 1.0mg·L-1。60 Co-γ射线诱变的半致死剂量(LD50)为24Gy,处理后的甜叶菊外观呈现显著差异,随着辐射剂量的增强,30d后甜叶菊死亡率分别为1.7%,2.4%,20.5%,92.7%,98.9%,99.4%,100%,辐射剂量是5、15、30Gy时,可溶性多糖含量较对照组分别增加76.77%,26.73%,15.29%,表明辐照诱变导致甜叶菊试管苗短期的应激效应。研究结果为甜叶菊的辐照诱变育种提供了理论参考。
In order to obtain the optimal medium for the proliferation of Stevia and the dose of 60 Co-γ radiation breeding, the stems of the sterile Stevia rebaudianae were used as explants, and the hormone type and concentration in the proliferation medium were optimized by orthogonal experiment On this basis, the stem segments of Stevia rebaudiana were irradiated by irradiation with 60Co-γ ray at doses of 5, 15, 30, 60, 90, 120 and 150 Gy respectively, the growth and the mortality were recorded every 15 days. The contents of soluble polysaccharides and soluble proteins in leaves treated with ≤30Gy were determined. The results showed that the optimal medium for the proliferation of stevia stems was MS + NAA 0.5 mg · L-1 + TDZ 0.1 mg · L-1 + 6-BA 1.0 mg · L-1.60 Co-γ-ray The LD50 was 24Gy and the appearance of stevia was significantly different. With the increase of radiation dose, the mortality of Stevia rebaudiana was 1.7%, 2.4%, 20.5%, 92.7%, 98.9% and 99.4 %, 100%. When the radiation dose was 5, 15 and 30 Gy, the content of soluble polysaccharides increased by 76.77%, 26.73% and 15.29% respectively compared with the control group, indicating that short-term stress effect of the stevia rebaudiana seedlings was induced by irradiation. The results provide a theoretical reference for the radiation induced mutation breeding of Stevia rebaudiana.