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目的探讨蒺藜皂苷(GSTT)后处理对大鼠心肌缺血再灌注损伤的保护作用。方法 56只大鼠随机分为假手术组、缺血再灌注模型组、缺血后处理组、阳性药尼可地尔1.0 mg/kg组、GSTT(100、30、10 mg/kg)组。采用结扎大鼠心脏左冠状动脉前降支的方法,制备心肌缺血再灌注损伤模型,分离血清,采用分光光度法测定乳酸脱氢酶(LDH)、丙二醛(MDA)水平和超氧化物歧化酶(SOD)的活性,ELISA法测定血清中肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的量。光学显微镜下观察受损心肌病理组织学改变,采用TUNEL法检测心肌细胞凋亡情况。结果与模型组比较,GSTT100、30 mg/kg组LDH、MDA、TNF-α和IL-6的量明显降低、SOD的活性明显升高(P<0.01),凋亡细胞数量明显减少(P<0.01),心肌组织形态明显改善。结论再灌注同时ipGSTT进行药物后处理对大鼠心肌缺血再灌注损伤具有保护作用,能减少自由基生成,抑制心肌细胞凋亡。
Objective To investigate the protective effect of GSTT on myocardial ischemia-reperfusion injury in rats. Methods 56 rats were randomly divided into sham operation group, ischemia / reperfusion model group, ischemic postconditioning group and nicorandil 1.0 mg / kg group, GSTT (100,30,10 mg / kg) group. The model of myocardial ischemia-reperfusion injury was established by ligation of left anterior descending coronary artery in rats. The serum was separated and the levels of lactate dehydrogenase (LDH), malondialdehyde (MDA) and superoxide Dismutase (SOD) activity was assayed by enzyme linked immunosorbent assay (ELISA). The levels of TNF-α and IL-6 in serum were measured by ELISA. Under the light microscope, the histopathological changes of damaged myocardium were observed. The apoptosis of cardiomyocytes was detected by TUNEL method. Results Compared with model group, the amounts of LDH, MDA, TNF-α and IL-6 in GSTT 100 and 30 mg / kg group were significantly decreased, the activity of SOD was significantly increased (P <0.01) 0.01), myocardial tissue morphology improved significantly. Conclusion Reperfusion and administration of ipGSTT have protective effect on myocardial ischemia-reperfusion injury, which can reduce the generation of free radicals and inhibit cardiomyocyte apoptosis.