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目的:研究胰岛素样生长因子(IGF)等对小肠黏膜前体细胞营养的作用。方法:通过不同体系的培养系统,分析不同的细胞因子对小肠黏膜前体细胞扩增和发育的影响。结果:IGF、肝细胞生长因子(HGF)、谷氨酰胺(G ln)等因素联合作用对体外培养的小肠黏膜前体细胞群体的数量有显著的促进作用,而IGF、HGF单独应用虽然可以有效扩增小肠黏膜前体细胞的数量,但相对于这些因子的联合应用而言,小肠黏膜细胞的体外扩增和发育受到一定的限制。G ln对小肠黏膜前体细胞在体内的发育有显著的促进作用,而对小肠黏膜前体细胞的体外扩增则无明显的作用。结论:IGF、HGF、G ln等因素联合作用可以有效解除有害细胞因素对小肠黏膜前体细胞的抑制,并提供小肠黏膜细胞生长发育的能量物质,而各因素的单独应用对小肠黏膜前体细胞的体外扩增和发育的作用是有限的。
Objective: To study the effect of insulin-like growth factor (IGF) on the intestinal mucosal precursor cells. Methods: The effects of different cytokines on the proliferation and development of intestinal mucosal precursor cells were analyzed by different system of culture system. Results: The combined effects of IGF, hepatocyte growth factor (HGF), glutamine and other factors had a significant effect on the number of small intestinal mucosal precursor cells cultured in vitro. While the application of IGF and HGF alone could be effective Expand the number of intestinal mucosa precursor cells, but compared to the combination of these factors, the expansion and development of small intestinal mucosal cells in vitro is subject to certain restrictions. Gln had a significant effect on the development of small intestine mucosal precursor cells in vivo, but had no significant effect on the ex vivo expansion of small intestinal mucosal precursor cells. Conclusion: The combined effects of IGF, HGF and Gln can effectively relieve the inhibition of small intestinal mucosal precursors by harmful cytokines and provide energy substances for the growth and development of intestinal mucosal cells. However, The role of in vitro expansion and development is limited.