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目的:观察全身垂直振动对去卵巢骨质疏松大鼠骨髓细胞分化的调节作用。方法:将36只3月龄雌性未孕SD大鼠按体重分层后随机分为假手术、去卵巢静止和去卵巢振动3个组。去卵巢手术后10周,去卵巢振动组大鼠经过1周适应振动治疗后,开始接受正式全身垂直振动治疗,每次振动15 min、频率为90 Hz,每天振动2次,每周7次。治疗7周时,用721分光光度计检测血清碱性磷酸酶和甘油三酯水平;常规HE染色观察左侧胫骨组织形态学;收集右侧股骨和胫骨骨髓细胞,一部分细胞用作碱性磷酸酶和油红O染色,另一部分细胞用作尼罗红(Nile Red)染色。结果:与假手术组比较,去卵巢静止组骨髓细胞碱性磷酸酶阳性染色细胞数目显著降低,而骨髓细胞Nile Red阳性染色细胞百分比和胫骨骨髓脂肪空泡数目显著增加;与去卵巢静止组比较,去卵巢振动组骨髓细胞碱性磷酸酶阳性染色细胞数目显著增加,而骨髓细胞Nile Red阳性染色细胞百分比和胫骨骨髓脂肪空泡数目显著下降。结论:全身垂直振动不仅增加去卵巢骨质疏松大鼠骨髓细胞的成骨分化能力,而且降低去卵巢骨质疏松大鼠骨髓细胞的成脂分化能力。
OBJECTIVE: To observe the regulation of whole body vertical vibration on differentiation of bone marrow cells of ovariectomized rats. Methods: Thirty-six 3-month-old female un-pregnant SD rats were randomly divided into sham operation group, ovariectomized group and ovariectomized group. After ovariectomy for 10 weeks, the ovariectomized rats began to undergo full-body vertical vibration therapy after one week of vibrational treatment. Each vibration for 15 min, the frequency of 90 Hz, vibration twice a day, 7 times a week. Serum ALP and triglyceride levels were measured by 721 spectrophotometer at 7 weeks after treatment. The morphology of the left tibia was observed by conventional HE staining. The right femur and tibial bone marrow cells were collected. A part of the cells were used as alkaline phosphatase And Oil Red O, and the other part was used as Nile Red staining. Results: Compared with the sham operation group, the number of alkaline phosphatase positive cells in the bone marrow cells of the ovariectomized group decreased significantly, while the percentage of Nile Red positive staining cells and the number of the adipose tissue in the tibial bone marrow significantly increased in the ovariectomized group. Compared with the static ovariectomy group , And the number of alkaline phosphatase positive cells in bone marrow cells in ovariectomized group increased significantly, while the percentage of Nile Red positive cells and the number of fat vacuoles in bone marrow of bone marrow decreased significantly. Conclusion: Vertical body vibration not only increases osteogenic differentiation of bone marrow cells in ovariectomized rats, but also decreases adipogenic differentiation of bone marrow cells in ovariectomized rats.