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目的探讨不同化学组成的纳米颗粒对人胃癌BGC-823细胞线粒体膜电位(mitochondrial membrane potential,MMP)及细胞内活性氧(reactive oxygen species,ROS)水平的影响。方法将不同浓度纳米活性炭(activated carbon nanoparticles,ACNP)、纳米二氧化硅(SiO2)、纳米二氧化钛(TiO2)作用于BGC-823细胞24h后,用流式细胞仪以罗丹明123(Rh123)作为荧光指示剂检测细胞MMP;用ROS捕获剂双氢罗丹明123孵育细胞,通过检测细胞内Rh123的平均荧光强度而测得细胞内ROS水平。结果经ACNP、纳米SiO2、纳米TiO2作用24h后,BGC-823细胞MMP呈剂量依赖性降低。0.1,0.2mg/mlACNP组细胞内ROS水平高于对照组(P<0.05);0.1,0.2,0.4mg/ml纳米SiO2组细胞内ROS水平呈剂量依赖性降低;0.1,0.2mg/ml纳米TiO2组细胞内ROS水平高于对照组(P<0.05)。结论 ACNP诱导细胞发生氧化应激,生成ROS,可能进一步通过活化线粒体信号转导途径诱导细胞凋亡;化学活性较强的纳米SiO2和纳米TiO2在含水介质中能够产生大量ROS,作用于细胞后能直接引起膜脂质过氧化,导致细胞膜破裂,细胞坏死。
Objective To investigate the effect of nanoparticles with different chemical compositions on the mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) levels in human gastric cancer BGC-823 cells. Methods After activated carbon nanoparticles (ACNP), nano-silica (SiO2) and nano-TiO2 were incubated with BGC-823 cells for 24 h at different concentrations, rhodamine 123 (Rh123) was used as fluorescence The MMPs were detected by the indicator reagent; ROS was detected by measuring the average fluorescence intensity of Rh123 in the cells by incubating the cells with the ROS capture DHT-123. Results After treated with ACNP, nano-SiO2 and nano-TiO2 for 24 h, MMPs in BGC-823 cells decreased in a dose-dependent manner. The level of ROS in 0.1,0.2mg / ml ACNP group was higher than that in the control group (P <0.05). The ROS level in 0.1,0.2,0.4 mg / ml nano-SiO2 group decreased in a dose-dependent manner. 0.1,0.2mg / ml nano- The level of intracellular ROS was higher than that of the control group (P <0.05). Conclusion ACNP induces cellular oxidative stress and generates ROS, which may further induce apoptosis through activation of mitochondrial signal transduction pathway. A large amount of ROS can be produced in aqueous medium by nano-SiO2 and nano-TiO2 with high chemical activity. Directly caused by membrane lipid peroxidation, leading to cell membrane rupture, cell necrosis.