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目的研究去甲氧基姜黄素羟丙基-β-环糊精(DECD)及去甲氧基姜黄素(DE)的在体肠吸收情况。方法使用DE以环糊精包合技术制备DECD,光谱法测定其理化性质,显微照相观察其形态,马尔文粒径测定仪测定DECD的zeta电位。采用大鼠在体肠段单向灌流模型,并建立紫外分光光度法测定DECD及DE在大鼠体内各肠段的吸收速率常数(Ka)、表观渗透系数(Papp)和吸收百分率。结果成功制得DECD,其溶解度为2.30g/L,是DE的38.33倍;zeta电位为-32.2mV。在体肠吸收实验显示DECD的Ka及Papp皆为回肠>十二指肠>空肠>结肠,且其Ka、Papp与吸收百分率较DE均有所提高。结论 DECD能够明显改善DE在大鼠小肠内的吸收情况。
Objective To study the in vivo intestinal absorption of demethoxycurcumin hydroxypropyl-β-cyclodextrin (DECD) and demethoxycurcumin (DE). Methods DED was prepared by cyclodextrin inclusion technique. The physicochemical properties were determined by spectrophotometry. The morphology was observed under photomicrograph. The zeta potential of DECD was determined by Malvern particle size analyzer. The unilateral rat perfused intestinal segment model was established and the absorption rate constant (Ka), apparent permeability coefficient (Papp) and absorption percentage of DECD and DE in various intestine segments of rats were determined by ultraviolet spectrophotometry. The results obtained successfully DECD, the solubility of 2.30g / L, 38.33 times the DE; zeta potential of -32.2mV. In vivo intestinal absorption experiments showed that the DEC and Ka were Papp and ileum> duodenum> jejunum> colon, and the Ka, Papp and the absorption percentage higher than DE. Conclusions DECD can obviously improve the absorption of DE in rat small intestine.