论文部分内容阅读
目的探究海地瓜硫酸软骨素(Acaudina Molpadioides chondroitin sulfate,AM-CHS)抑制3T3-L1前脂肪细胞分化的分子机制。方法采用传统的鸡尾酒诱导剂诱导分化3T3-L1前脂肪细胞,分别采用油红O染色和甘油三酯(Triglycerides,TG)含量测定法评价AM-CHS对3T3-L1前脂肪细胞分化的影响。采用RT-PCR法检测脂肪细胞中Wnt信号通路关键基因和脂肪合成关键基因mRNA的表达水平。结果AMCHS能够显著抑制3T3-L1细胞脂滴的形成,拮抗罗格列酮(Rosiglitazone,RSG)的促分化作用,并上调Wnt信号通路中的关键受体Frz和LRP5的mRNA表达,下调分化转录因子PPARγ、C/EBPα和SREBP-1cmRNA的表达,抑制脂质合成基因FAS和GPAT mRNA的表达,不影响脂质分解基因HSL和ATGL mRNA的表达。结论AM-CHS能够显著抑制3T3-L1前脂肪细胞的分化,抑制成熟脂肪细胞的脂质合成,并拮抗罗格列酮(Rosiglitazone,RSG)的促脂质合成作用,其作用机制与激活Wnt信号通路有关。
Objective To investigate the molecular mechanism of inhibition of 3T3-L1 preadipocyte differentiation by Acaudina molpadioides chondroitin sulfate (AM-CHS). Methods 3T3-L1 preadipocytes were induced by traditional cocktail inducer. The effects of AM-CHS on the differentiation of 3T3-L1 preadipocytes were evaluated by oil red O staining and triglyceride (TG) assay. RT-PCR was used to detect the mRNA expression of key genes of Wnt signaling pathway and adipogenesis in adipocytes. Results AMCHS could significantly inhibit the lipid droplets formation, antagonize the differentiation of Rosiglitazone (RSG) and up-regulate the mRNA expression of the key receptors (Frz and LRP5) in Wnt signaling pathway, downregulate the transcription factor PPARγ, C / EBPαand SREBP-1cmRNA, and inhibited the expression of lipid synthesis genes FAS and GPAT mRNA, but did not affect the expression of lipolytic genes HSL and ATGL mRNA. Conclusion AM-CHS can significantly inhibit the differentiation of 3T3-L1 preadipocytes, inhibit the lipid synthesis of mature adipocytes and antagonize the lipid-lipid synthesis of Rosiglitazone (RSG), and its mechanism of action and activation of Wnt signaling Path related.