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为研究基因重组乙肝疫苗对HBV转基因小鼠的免疫调节作用,取经HBsAg免疫的小鼠脾淋巴细胞,制成5×106细胞悬液,分两份,一份经无佐剂HBsAg体外刺激48h后,取上清检测细胞因子;另一份细胞于培养56h后加入3H-TdR,继续培养16h,测定cpm值。结果表明,转基因鼠经多次免疫8个月后,其脾细胞产生的Th1类细胞因子(IL-2、IFN-γ)及T细胞增殖水平均较单次免疫一周后有显著性提高。而采用不同剂量HBsAg按常规免疫时并不能提高IL-2的水平。结果提示,反复大剂量的HBsAg可以打破HBV转基因小鼠的免疫耐受,上调其细胞免疫功能,这为慢性乙肝的临床治疗提供了实验根据。
In order to study the immunomodulatory effect of recombinant hepatitis B vaccine on HBV transgenic mice, 5 × 10 6 cell suspension was prepared from the spleen lymphocytes of mice immunized with HBsAg in two replicates. One of them was stimulated in vitro with unadjuvanted HBsAg for 48 h , And the supernatant was used to detect cytokines. Another cell was added to 3H-TdR after 56h of culture, and cultured for 16h. The cpm value was measured. The results showed that the level of Th1 cytokines (IL-2, IFN-γ) and T cell proliferation in spleen cells of transgenic mice after 8-month immunization was significantly higher than that of the one-week immunization. The use of different doses of HBsAg routine immunization does not improve the level of IL-2. The results suggest that repeated high doses of HBsAg can break the immune tolerance of HBV transgenic mice and upregulate their cellular immune function, which provides experimental evidence for the clinical treatment of chronic hepatitis B.